What are the differences between isoschizomers and neoschizomers enzymes?

Isoschizomers are restriction enzymes that have the same recognition sequence and the same specificity. … Neoschizomers recognize the same nucleotide sequence but cleave DNA at different positions.

What do you mean by neoschizomers?

Neoschizomers are restriction enzymes that recognize the same nucleotide sequence as their prototype but cleave at a different site. In some special applications this is a very helpful feature. … Neoschizomers are a subset of isoschizomers.

What is the difference between an endonuclease and exonuclease enzyme?

The main difference between these enzymes is that endonucleases cleave the phosphodiester bond in the polynucleotide present internal in the polynucleotide chain, whereas exonucleases cleave the phosphodiester bond from the ends.

How do you identify an isoschizomer?

Isoschizomers with alternative cleavage sites are indicated with a " ^ ". Enzymes that are not currently commercially available are indicated with a " x ". Neoschizomers are a subset of isoschizomers that recognize the same sequence, but cleave at different positions from the prototype.

What are isoschizomers and give its example?

Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other. … For example, the restriction enzymes HpaII and MspI are isoschizomers, as they both recognize the sequence 5'-CCGG-3' when it is unmethylated.

Does EcoRI have isoschizomers?

Isoschizomers | Single Letter Code | Pronunciation: Time-Saver™ qualified for digestion in 5-15 minutes. High Fidelity (HF®) version available (NEB #R3101) supplied with rCutSmart™ Buffer.

What is XmaI?

XmaI is an isoschizomer of SmaI. XmaI produces a 5´ extension whereas SmaI produces blunt-ended fragments. Impaired by CpG methylation. star activity may result from a glycerol concentration of >5%